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豚鼠(shu)端(duan)粒(li)酶(mei) ELISA試劑盒(he) 酶聯(lian)免疫(yi)吸附(fu)測定(ding)試劑盒(he)使用(yong)說明(ming)書(shu)elisa法(fa)測(ce)定(ding)規格(ge):48T/96T本(ben)試劑盒(he)僅供體(ti)外研(yan)究使用(yong)!ELISA法定量測定(ding)人(ren)尿液或其(qi)它相關(guan)生物(wu)液體(ti)中的含量。
實驗(yan)原理(li)用純(chun)化(hua)的抗(kang)體(ti)包被(bei)微孔(kong)板(ban),制成(cheng)固(gu)相(xiang)載體(ti),往包被(bei)抗(kang)指(zhi)標(biao)抗(kang)體(ti)的微孔(kong)中(zhong)依次(ci)加入標(biao)本(ben)或標準(zhun)品(pin)、化(hua)的抗(kang)指(zhi)標(biao)抗(kang)體(ti)、HRP標記(ji)的親和素,經(jing)過(guo)*洗(xi)滌後(hou)用底(di)物(wu)TMB顯(xian)色(se)。TMB在過(guo)氧(yang)化(hua)物酶(mei)的催化(hua)下轉化(hua)成藍色,並在酸(suan)的作用下轉化(hua)成zui終(zhong)的黃色。顏(yan)色(se)的深淺(qian)和樣品(pin)中的指標呈正(zheng)相(xiang)關(guan)。用酶標(biao)儀(yi)在450nm波(bo)長下(xia)測定(ding)吸光(guang)度(OD值(zhi)),計算(suan)樣(yang)品(pin)濃(nong)度。豚鼠(shu)端(duan)粒(li)酶(mei) ELISA試劑盒(he) 試劑盒(he)組(zu)成(cheng)及(ji)試劑配制(zhi).試劑盒(he)保存(cun):-20℃(較(jiao)長時(shi)間(jian)不(bu)用時(shi));2-8℃(頻(pin)繁使用(yong)時(shi))。2.濃(nong)洗(xi)滌液低溫保存(cun)會(hui)有鹽(yan)析出,稀釋時(shi)可(ke)在(zai)水浴中(zhong)加溫助(zhu)溶(rong)。3.中(zhong)、英(ying)文說明(ming)書(shu)可(ke)能(neng)會(hui)有不(bu)*之處,請(qing)以(yi)英(ying)文說明(ming)書(shu)為(wei)準。4.剛(gang)開啟的酶聯板孔(kong)中(zhong)可(ke)能(neng)會(hui)含有少(shao)許(xu)水樣物(wu)質,此為(wei)正(zheng)常現象,不(bu)會(hui)對(dui)實(shi)驗(yan)結(jie)果造(zao)成(cheng)任何影(ying)響。需要而未(wei)提(ti)供的試劑和器材(cai). 標(biao)準規格(ge)酶標(biao)儀(yi)2. 高速(su)離(li)心(xin)機(ji)3. 電(dian)熱(re)恒溫培養箱4. 幹凈(jing)的試管(guan)和Eppendof管(guan)5. 系列(lie)可(ke)調節移液器及(ji)吸(xi)頭,壹次(ci)檢(jian)測樣(yang)品(pin)較(jiao)多時(shi),用(yong)多通(tong)道移(yi)液(ye)器(qi)6. 蒸(zheng)餾水,容量瓶等(deng)
. 酶聯(lian)板(Assay plate ):壹塊(96孔(kong))。2. 標(biao)準品(pin)(Standard):2瓶。3. 樣(yang)品(pin)稀釋液(ye)(Sample Diluent):×20ml/瓶。4. 標(biao)記(ji)抗(kang)體(ti)稀釋液(ye)(Biotin-antibody Diluent):×0ml/瓶。5. 辣(la)根(gen)過(guo)氧(yang)化(hua)物酶(mei)標記(ji)親(qin)和素稀釋液(ye) (HRP-avidin Diluent):×0ml/瓶。6. 標(biao)記(ji)抗(kang)體(ti)(Biotin-antibody):×20μl/瓶(:00)7. 辣(la)根(gen)過(guo)氧(yang)化(hua)物酶(mei)標記(ji)親(qin)和素(HRP-avidin):×20μl/瓶(:00)8. 底(di)物溶(rong)液(ye)(TMB Substrate):×0ml/瓶。9. 濃(nong)洗(xi)滌液(Wash Buffer):×20ml/瓶,使用(yong)時(shi)每(mei)瓶用(yong)蒸(zheng)餾水稀釋25倍(bei)。0. 終(zhong)止液(ye)(Stop Solution):×0ml/瓶(2N H2SO4)。
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